解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:OBJECTIVES:To determine the effectiveness of evoglow-Pp1 as a reporter to study gene expression in bifidobacteria. To choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions. RESULTS:The elongation factor P (EF-P) promoter from Bifidobacterium...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1802-8
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:To evaluate the therapeutic potential of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) on promoting erectile function in a rat model of bilateral cavernous nerve (CN) crush injury. RESULTS:Fifty male Sprague-Dawley rats were randomly assigned to sham + PBS group (n = 10), BCNI (bilateral caver...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1816-2
更新日期:2015-07-01 00:00:00
abstract::Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1786-4
更新日期:2015-06-01 00:00:00
abstract::Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1764-2
更新日期:2015-05-01 00:00:00
abstract::Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ''amino acid adenylation domain'' located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specificall...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1766-8
更新日期:2015-05-01 00:00:00
abstract::The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1751-7
更新日期:2015-04-01 00:00:00
abstract::Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1735-7
更新日期:2015-04-01 00:00:00
abstract::In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1730-z
更新日期:2015-03-01 00:00:00
abstract::The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1709-9
更新日期:2015-03-01 00:00:00
abstract::A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1721-0
更新日期:2015-03-01 00:00:00
abstract::A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1675-2
更新日期:2015-02-01 00:00:00
abstract::An alginate lyase gene, algA, encoding a new poly β-D-mannuronate (polyM)-specific alginate lyase AlgA, was cloned from Pseudomonas sp. E03. The recombinant AlgA with (His)6-tag, consisting of 364 amino acids (40.4 kDa),was purified using Ni-NTA Sepharose. The purified lyase had maximal activity (222 EU/mg) at pH 8 an...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1685-0
更新日期:2015-02-01 00:00:00
abstract::The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1691-2
更新日期:2015-02-01 00:00:00
abstract::Microbes employ cell membranes for reducing exogenous stresses. Polyamino acid display on microbial cell surfaces and their effects on microbial chemical stress tolerance were examined. Growth analysis revealed that displays of polyarginine, polyaspartate and polytryptophan substantially enhanced tolerance of Escheric...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1689-9
更新日期:2015-02-01 00:00:00
abstract::A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a G...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1648-5
更新日期:2015-01-01 00:00:00
abstract::Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. (R)-1,2-Propanediol (>99 % e.e.) was obtained at 44 % yield with gr...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1670-7
更新日期:2015-01-01 00:00:00
abstract::Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1631-1
更新日期:2014-12-01 00:00:00
abstract::Theabrownins (TB) are water-soluble phenolic compounds associated with the various health benefits of Pu-erh tea, a post-fermented Chinese dark tea. This work reports on the production of theabrownins from infusions of sun-dried green tea leaves using a pure culture of Aspergillus fumigatus isolated from a solid-state...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1632-0
更新日期:2014-12-01 00:00:00
abstract::The thermostablility and enzymatic activity of 1,3-1,4-β-glucanase (BglA) from Bacillus amyloliquefaciens was improved by modifying five (out of 12) ε-amino groups in lysine residues with nitrous acid. The optimal modification condition for BglA was determined as 30 mM nitrous acid at, 40 °C for 30 min. The optimally-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1616-0
更新日期:2014-12-01 00:00:00
abstract::Microcycle conidiation is a survival mechanism of fungi encountering unfavorable conditions. In this phenomenon, asexual spores germinate secondary spores directly without formation of mycelium. As Penicillium camemberti conidia have the ability to produce conidiophores after germination in liquid culture induced by a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1596-0
更新日期:2014-11-01 00:00:00
abstract::The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD(+) as a cofactor. Although NAD(+)-dependent ALDH overexpression facilitates 3-HP biosynthesis...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1590-6
更新日期:2014-11-01 00:00:00
abstract::β-Xylosidase from Thermoanaerobacterium thermosaccharolyticum was purified by His-trap affinity chromatography giving a specific activity of 5.15 U mg(-1). From gel-filtration chromatography, the purified enzyme was a tetramer with a total molecular mass of 245 kDa. Maximal enzyme activity using o-nitrophenyl(NP)-β-D-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1604-4
更新日期:2014-11-01 00:00:00
abstract::Ammonium is a substrate of the anaerobic ammonium oxidation (Anammox) process but it has been suggested as a substrate-inhibitor because of the action of its unionized form, free ammonia. High pH of the medium is also an important limiting factor of the Anammox bacteria. Both effects are difficult to discriminate. In ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1564-8
更新日期:2014-10-01 00:00:00
abstract::Targeted gene replacement in the thermotolerant yeast Kluyveromyces marxianus KCTC 17555 has been hampered by its propensity to non-homologous end joining (NHEJ). To enhance homologous recombination (HR) by blocking NHEJ, we identified and disrupted the K. marxianus KU80 gene. The ku80 deletion mutant strain (Kmku80∆)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1576-4
更新日期:2014-10-01 00:00:00
abstract::In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1581-7
更新日期:2014-10-01 00:00:00
abstract::Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1546-x
更新日期:2014-09-01 00:00:00
abstract::Biodesulfurization (BDS) in a bioreactor packed with a catalytic bed of silica containing immobilized Rhodococcus rhodochrous was studied. Various bed lengths and support particle sizes were evaluated for BDS of dibenzothiophene (DBT) and gas oil. The sulfur-containing substrates were introduced separately into the bi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1529-y
更新日期:2014-08-01 00:00:00
abstract::Nitrogenous derivatives of the two orange pigments from Monascus sp. with anti-melanogenic activities were prepared using fermentation and chemical synthesis. The pigments were produced in a 5 l jar fermentor. A total of 33 derivatives were synthesized via incorporation of L-amino acids and amines into the pigments. T...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1518-1
更新日期:2014-08-01 00:00:00
abstract::KGLP-1, a 31-amino acid glucagon-like peptide-1 (GLP-1) analogue, has a great therapeutic potential for anti-diabetes. In this work, a strategy for expression and purification of functional KGLP-1 peptide has been established. KGLP-1 cDNA was fused with glutathione S-transferase (GST), with an enterokinase cleavage si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1526-1
更新日期:2014-08-01 00:00:00
abstract::Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1501-x
更新日期:2014-07-01 00:00:00
abstract::In this study, 3D culture system for human adipose-derived stem cell (hASC) using a BioLevitator as the bioreactor for microcarrier-based cultures was established. During the culturing period, hASCs preferred to grow in crevices between microcarriers and a high viability was maintained even when reaching confluency. A...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1500-y
更新日期:2014-07-01 00:00:00
abstract::The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped shar...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1467-8
更新日期:2014-06-01 00:00:00
abstract::General methods for identification of usual carotenoids including C18-HPLC retention time and spectroscopic methods, such as absorption spectra, mass spectra, NMR spectra, are briefly summarized. ...
journal_title:Biotechnology letters
pub_type: 信件
doi:10.1007/s10529-014-1479-4
更新日期:2014-06-01 00:00:00
abstract::To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1489-2
更新日期:2014-06-01 00:00:00
abstract::Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detecta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1455-z
更新日期:2014-05-01 00:00:00
abstract::Sodium butyrate is commonly used in mammalian cell cultures to increase the productivity of recombinant proteins. A Chinese hamster ovary (CHO) cell line producing recombinant human erythropoietin (rhEPO) was cultured in commercial medium. Addition of 0.5 mM butyrate inhibited the over-growth of the cells after the me...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1442-9
更新日期:2014-05-01 00:00:00
abstract:PURPOSE OF WORK:The purpose of this study was to determine if Arabidopsis protoplast transfection could be scaled up, from the commonly used cell-based studies, to be used in triterpenoid production assays as an in planta alternative/complement to other expression systems. Enzyme activities are often identified using h...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1427-8
更新日期:2014-04-01 00:00:00
abstract::A novel gene encoding the α-chain of goose immunoglobulin heavy-chain (Igα) was cloned by reverse transcription-PCR. The cDNA had 1,760 bp and encompassed a partial V-D-J region of the heavy chain, a constant region (Cα) and 3'-untranslated region of α-chain. The Cα gene contains four constant region domains (CH1-CH4)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1415-z
更新日期:2014-04-01 00:00:00
abstract::We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1400-6
更新日期:2014-03-01 00:00:00
abstract::The shape of the hydrophobic tunnel leading to the active site of Penicillium expansum lipase (PEL) was redesigned by single-point mutations, in order to better understand enzyme enantioselectivity towards naproxen. A variant with a valine-to-glycine substitution at residue 237 exhibited almost no enantioselectivity (...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1405-1
更新日期:2014-03-01 00:00:00